Lecture 01: Introduction

Preview 35:55

Lecture 02: Next Generation Sequencing Technologies - 454 Sequencing

Preview 30:36

Lecture 03: Illumina Sequencing By Synthesis (SBS)

Preview 32:28

Lecture 04: Single Molecule Real Time (SMRT) Sequencing

Preview 31:10

Lecture 05: Ion Torrent and Nanopore Sequencing

Preview 32:42

Lecture 06: Sequencing Coverage,Quality Score and Experiment Design

Preview 30:51

Lecture 07 : Data Formats

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Lecture 08 : Data Formats (Contd.)

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Lecture 09 : Data Quality

Preview 27:28

Lecture 10: Data QC and Trimming

Preview 29:35

Lecture 11 : Hands on: Setting up the system

Preview 33:45

Lecture 12: Basic Shell Commands

Preview 32:45

Lecture 13: Data Download and Exploration

Preview 31:42

Lecture 14: Hands-on 1 - Data exploration and QC

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Lecture 15 : Hands-on 1 - Data QC and Trimming

Preview 33:42

Lecture 16 : Read Mapping

Preview 31:03

Lecture 17 : Mapping Algorithms

Preview 33:36

Lecture 18 : Suffix tree-based mapping algorithm

Preview 32:11

Lecture 19 : Burrows-Wheeler Transform (BWT)

Preview 30:58

Lecture 20 : Read Mapping with BWT

Preview 30:56

Lecture 21 : Bowtie2 tool

Preview 32:22

Lecture 22 : Mapping reads with Bowtie2

Preview 33:41

Lecture 23: Bowtie2 output

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Lecture 24 : SAM and BAM format

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Lecture 25 : SAM format: Alignment section

Preview 34:33

Lecture 26: Variant Calling

Preview 28:54

Lecture 27: Calling SNP/SNVs and Indels

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Lecture 28: Hands-on analysis : Variant Calling

Preview 39:15

Lecture 29 : VCF Files

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Lecture 30: Variant Annotation

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Lecture 31 : Analysis of CNVs and SVs

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Lecture 32 : Introduction to RNA sequencing

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Lecture 33 : RNA-seq data processing pipeline

Preview 34:30

Lecture 34 : Transcriptome Assembly & Quantification

Preview 29:47

Lecture 35 : Transcript Abundance Quantification

Preview 31:19

Lecture 36 : Biases in RNA-seq experiments

Preview 33:36

Lecture 37 : Data Normalization Methods

Preview 32:28

Lecture 38 : Data Normalization Methods (Contd.)

Preview 32:03

Lecture 39 : Differential Gene Expression (DGE) Analysis

Preview 33:10

Lecture 40 : DGE analysis results and visualizations

Preview 35:16

Lecture 41 : Multiple hypothesis testing correction

Preview 30:30

Lecture 42 : FDR correction and interpretation of DGE analysis results

Preview 30:14

Lecture 43 : Functional Enrichment Analysis

Preview 32:49

Lecture 44 : RNA-seq data analysis – Hands-on 2

Preview 31:33

Lecture 45 : Hands-on 2: Setting up the system

Preview 31:19

Lecture 46 : Hands-on 2: Preliminary Data Analysis

Preview 33:22

Lecture 47 : Sample Specific Bias Correction

Preview 31:59

Lecture 48 : Differential Gene Expression Analysis I

Preview 33:53

Lecture 49 : DGE Analysis with spike-ins

Preview 33:18

Lecture 50 : DGE Analysis Results and Functional Enrichment Analysis

Preview 32:37

Lecture 51 : Genome Assembly

Preview 30:37

Lecture 52 : Shortest Common Superstring (SCS) assembly

Preview 31:27

Lecture 53 : Overlap-Layout-Consensus (OLC) approach

Preview 32:03

Lecture 54 : de Bruijn Graph (DBG) based assembly

Preview 30:20

Lecture 55 : Assembly and Quality Control

Preview 34:24

Lecture 56 : Applications of NGS in Epigenomics

Preview 31:29

Lecture 57 : Detecting DNA Methylations

Preview 31:10

Lecture 58 : Genome-wide Transcription Factor(TF) Binding Sites

Preview 30:23

Lecture 59 : Chromatin Accessibility

Preview 27:49

Lecture 60 : Genome Organization in 3D

Preview 31:25

Live Session 17-10-2023

Preview 58:50

Live Session 18-09-2023

Preview 56:50

Live Session 24-08-2023

Preview 52:59